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2.2. Transgenic plant generation ABI5-binding protein 4. TAIR , Complete GO annotation on QuickGO Keywords - Cellular component i Nucleus. Pathology & Biotech i Disruption phenotype i abi5 Encodes a member of the basic leucine zipper transcription factor family, involved in ABA signalling during seed maturation and germination.

ABI5 is able to form homo- or heterodimers with other ABF/AREB proteins. For instance, ABI5 was shown to interact with ABF3 and ABF1 (Kim et al., 2002; Finkelstein et al., 2005). 1994-10-01 · Interacts (via C-terminus) with SPK1, SCAR3, ABI5, APRR1, AIP2, AIP3 and AIP4. Binds to BZIP10 and BZIP25 and forms complexes made of ABI3, BZIP53 and BZIP25 or BZIP10.

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TAIR Curator Summary Encodes a member of the basic leucine zipper transcription factor family, involved in ABA signalling during seed maturation and germination. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. negative regulation of seed germination Source: TAIR "The gibberellic acid signaling repressor RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis and ABI5 activity." Piskurewicz U. , Jikumaru Y. , Kinoshita N. , Nambara E. , Kamiya Y. , Lopez-Molina L. Plant Cell 20:2729-2745(2008) [ PubMed ] [ Europe PMC Background ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters.

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Transgenic plant generation TheABI4-1345to0andABI5-1097to0promotersequenceswere Therefore, the expression of ABI5, MYB30 and their target genes were evaluated in asp1 and wild‐type seedlings. Expression of ABI5 and ABI5‐target genes, RD29A, RD29B, RAB18, Em6, and ADH1, were induced by exogenous ABA treatment, but the induction was less abundant in asp1 than in wild‐type seedlings (Figure 3A). ABI5-BINDING PROTEIN2 (AFP2) negatively regulates the abscisic acid signal by accelerating ABI5 degradation during seed germination in Arabidopsis (Arabidopsis thaliana).

The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. Product no AS12 1863 ABI5 | Abscisic acid insensitive 5 (peptide antibody) Product information Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana ABI5 sequence,UniProt: Q9SJN0, TAIR: AT2G36270 Host Rabbit Clonality Polyclonal Purity Affinity purified serum in PBS, pH 7.4 Format Lyophilized Quantity 100 µg Reconstitution For reconstitution add 100 µl of sterile water to each tube.
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ABF2, GeneProduct, AT1G45249 (TAIR), Related to flowering; no pathway ABI5, GeneProduct, AT2G36270 (TAIR), Projected from TAIR, homologous to LOC_Os01g64000 (ABI5). ADG1, GeneProduct, AT5G48300 (TAIR). Projected The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. Comparison of seed and ABA-inducible vegetative gene expression in wild-type and abi5-1 plants indicates that ABI5 regulates a subset of late embryogenesis-abundant genes during both developmental stages.
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Acts as a negative regulator of abscisic acid signaling. Required for ABI5 degradation, by mediating its ubiquitination. Together with EDR1, may regulate endocytic trafficking and/or the formation of signaling complexes on trans-Golgi network (TGN)/ early endosome (EE) vesicles during stress responses.